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Boster Bio
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Microtubule-associated proteins (MAPs) regulate microtubule stability and play critical roles in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP-light chain 3 beta (MAP-LC3 Beta) and MAP-light chain 3 alpha (MAP-LC3
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Image Search Results
Journal: Frontiers in Oncology
Article Title: CDK1-driven phosphorylation networks promote glioblastoma progression via MAP1B-mediated microtubule destabilization
doi: 10.3389/fonc.2025.1646698
Figure Lengend Snippet: CDK1-mediated MAP1B phosphorylation is positively correlated with the prognosis of GBM. (A) Overlap analysis of CPTAC phosphoproteome data (blue) with CDK1-regulated phosphosites (red). (B) Heatmap of MAP1B phosphosites in the shCDK1 and shCtrl groups. Red indicates upregulated values relative to the mean, while blue indicates downregulated values relative to the mean. The intensity of the color corresponds to the magnitude of the deviation. (C) Prognostic significance of MAP1B phosphorylation sites. Kaplan-Meier survival analysis of GBM patients stratified by phosphorylation levels at S832, S1260, S1899, S1939, S2209, S2271(log-rank test, p < 0.05). High phosphorylation correlates with poor overall survival (n=99). (D, E) CoIP showing interactions between CDK1 and MAP1B. (F) Detecting pS/T phosphorylation of MAP1B in shCDK1 and shCtrl U251 cells. (G) Detecting pS/T phosphorylation of MAP1B in overexpressed-CDK1(OE-CDK1) and empty vector(Vector) U251 cells.
Article Snippet: Phospho-(Ser/Thr) Phe Antibody (#9631, Cell Signaling Technology),
Techniques: Phospho-proteomics, Plasmid Preparation
Journal: Frontiers in Oncology
Article Title: CDK1-driven phosphorylation networks promote glioblastoma progression via MAP1B-mediated microtubule destabilization
doi: 10.3389/fonc.2025.1646698
Figure Lengend Snippet: CDK1-mediated phosphorylation of MAP1B regulates microtubule stability in U251 cells. (A) Wound healing assays showing delayed gap closure in shMAP1B cells compared to shCtrl cells over 36 hours, ***p < 0.001 (t-test), ****p < 0.0001 (t-test). (B) Transwell migration assays confirmed the impaired migration ability of shMAP1B cells. ***p < 0.001 (t-test). (C) CCK-8 proliferation assays revealed a significant decrease in cell viability in shMAP1B cells ***p < 0.001 (t-test). (D) Wiki-pathways analysis of differently expressed phosphoproteins. (E) Representative photographs of alpha tubulin staining of in U251 cells. Scale bar: 20 μm. Cells were stained with an antibody against acetyl-α-tubulin (green). Nuclei were counterstained with DAPI (blue). Scale bar, 20 μm.
Article Snippet: Phospho-(Ser/Thr) Phe Antibody (#9631, Cell Signaling Technology),
Techniques: Phospho-proteomics, Migration, CCK-8 Assay, Staining
Journal: Behavioral and Brain Functions : BBF
Article Title: Astragalus injection protects cerebral ischemic injury by inhibiting neuronal apoptosis and the expression of JNK3 after cerebral ischemia reperfusion in rats
doi: 10.1186/1744-9081-9-36
Figure Lengend Snippet: The expression of JNK3 in parahipppocampal area, SP staining × 400. (A) Control group: neurons arranged neatly, cell structure was completely. (B) Model group: neurons arranged disorderly, the number of JNK3-positive neurons increased significantly. (C) Treatment group: the number of JNK3-positive neurons (→) decreased significantly.
Article Snippet: The
Techniques: Expressing, Staining, Control
Journal: Behavioral and Brain Functions : BBF
Article Title: Astragalus injection protects cerebral ischemic injury by inhibiting neuronal apoptosis and the expression of JNK3 after cerebral ischemia reperfusion in rats
doi: 10.1186/1744-9081-9-36
Figure Lengend Snippet: The fragmentation formed by JNK3 protein detected by Western blotting. Line 1: a weaker fragment was formed by JNK3 protein in control group. Line 2: a strong fragment was formed by JNK3 protein in model group. Line 3: a middle fragment was formed by JNK3 protein in treatment group.
Article Snippet: The
Techniques: Western Blot, Control
Journal: Behavioral and Brain Functions : BBF
Article Title: Astragalus injection protects cerebral ischemic injury by inhibiting neuronal apoptosis and the expression of JNK3 after cerebral ischemia reperfusion in rats
doi: 10.1186/1744-9081-9-36
Figure Lengend Snippet: Electrophoresis analysis of RT-PCR products of JNK3 mRNA (left) and GAPDH (right). M: Maker; Line 1–2: weak light bands in control group; Line 3–4: strong light bands in model group; Line 5–6: middle light bands in treatment group
Article Snippet: The
Techniques: Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Control